K25-610022

MEIO GC

Descrição do produto

Aplicação:

É suplementado com suplementos seletivos e de enriquecimento é utilizado para isolar e cultivar gonococos, meningococos e Haemophilus spp.

Preparação:

– Suspender 43.0g do pó em 1 litro de água destilada ou deionizada. Aquecer até dissolver completamente.

– Esterelizar em autoclave a 121°C por 15 minutos. Resfriar 45-50°C.

– Adicionar 5-10% de sangue de cavalo desfibrinado no meio assepticamente esterelizado e aquecer em banho maria controlando a temperatura a 80°C por 15 minutos.

– Resfriar a 45-50°C e adicionar 2 frascos de Suplemento de Crescimento Vitalex (código K25-81023) reconstituído com 5mL de solução  restauradora Vitalex. Além disso, adicionar 2 frascos de Suplemento V.C.N (código K25-81022) reconstituído com 5mL de água destilada, a fim de obter um meio seletivo para Neisseria.

– Para análise das amostras de origens retal pode-se adicionar o Suplemento V.C.N.T (código K25-81024) ao invés do Suplemento V.C.N, a fim de inibir o crescimento excessivo de Proteus spp.

– Adicionar 2 frascos de SuplementoHaemophilus (código K25-81014), reconstituído com 5mL de água destilada a fim de obter um meio seletivo para Haemophilus. Preparar o meio como indicado e em seguida colocar em placas de Petri.

Especificações Técnicas:

Composição do Meio GC (g/l):

  • Peptona Proteose n°3 – 15.0
  • Amido de Milho – 1.0
  • Fosfato de Potássio Dibásico – 4.0
  • Fosfato de Potássio Monobásico – 1.0
  • Cloreto de Sódio – 5.0
  • Agar – 17.0

Composição do Suplemento V.C.N  mg/frasco):

  • Vancomicina – 1.5mg
  • Sulfato de Colistina – 3.75mg
  • Nistatina 6250 uL

*1 Frasco para 500mL do meio.

Composição Suplemento de Crescimento Vitalex (mg/frasco):

  • Vitamina B12 – 0.1mg
  • L-Glutamina – 100.0mg
  • Adenina SO4 – 10.0mg
  • Guanina HCL – 0.3mg
  • Ácido p-Aminobenzóico – 0.13mg
  • Coenzima 1 (NAD) – 2.5mg
  • Cocarboxilase – 1.0mg
  • Nitrato Férrico – 0.2mg
  • Hidrocloreto de Tiamina – 0.03mg
  • Hidrocloreto de Cisteína – 259.0mg

Composição da Solução Restauradora Vitalex (mg/frasco):

  • Glicose – 0.5mg
  • Água Destilada – 5.0mL 

*1 Frasco para 500mL do meio.

Composição do Suplemento  Haemophilus (mg/frasco):

  • Bacitracina – 150.0mg
  • Vancomicina – 5.0mg
  • Clindamicina – 0.5mg

  *1 Frasco para 500mL do meio.

Composição do Suplemento L.C.A.T  (mg/frasco):

  • Lincomicina – 0.5mg
  • Sulfato de Colistina – 3.0mg
  • Anfotericina B – 0.5mg
  • Trimetoprim – 3.25mg

*1 Frasco para 500mL do meio.

Composição do Suplemento V.C.A.T (mg/frasco):

  • Vancomicina – 1.0mg
  • Sulfato de Colistina – 3.75mg
  • Anfotericina B – 0.5mg
  • Trimetoprim – 3.25mg

*1 Frasco para 500mL do meio.

pH Final 7.2 + 0.2 a 25°C

 

Aparência do Meio:

Desidratado: Pó bege, fluído e homogêneo.

Preparado: Pó marrom-chocolate e opaco.

Controle de Qualidade:

Condições de Incubação: 36 ± 1°C por 24-48 horas, 5-10% CO2, meio basal.

 

Microrganismos                               ATCC           Crescimento

Neisseria gonorrhoeae                  43069                 Bom

Haemophilus influenzae               10211                 Bom

Condições de Incubação: 36 ± 1°C por 24-48 horas, meio basal + Suplemento V.C.N., V.C.N.T.,V.C.A.T. ou L.C.A.T.

 

Microrganismos                           ATCC            Crescimento

Neisseria gonorrhoeae              43069                  Bom

Neisseria meningitidis               10211                   Bom

Staphylococcus aureus              25923                  Inibido parcialmente

Condições de Incubação: 36 ± 1°C por 24-48 horas, 5-10% de CO2, meio basal + Suplemento Haemophilus.

Microrganismos                           ATCC           Crescimento

Enterococcus faecalis                 43069                Bom

Haemophilus influenzae            10211                Bom

 

Armazenamento:

O pó é muito higroscópico: Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os sinais de deterioração ou contaminação sejam evidentes. Armazenar em placas preparadas entre 2-8°C.

 

Descarte:

Não descartar em esgotos ou lixos domésticos. O produto deve ser tratado conforme as regulações oficiais administrativas. Soluções em excesso e não recicláveis destinar para empresa responsável. As embalagens contaminadas devem ser eliminadas como produto não utilizado.

Registro ANVISA:

Não aplicável.

Apresentação:

Frasco com 500 gramas.

 

MODELO: K25-610022

MARCA: KASVI

Sinônimos: GC Medium, GC Agar Base, Thayer- Martin Agar Base.

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