K25-610009

AGAR VERDE BRILHANTE

Descrição do produto

Aplicação

O Agar Verde Brilhante é um meio seletivo e adequado para o isolamento de Salmonella spp., exceto a Salmonella typhi, que é encontrada em  fezes, produtos lacticinios, ou outros alimentos suspeitos.

Preparação:

Suspender 58.1g de pó em 1 litro de água destilada ou deionizada. Aquecer até dissolver completamente. Esterilizar em autoclave a 121°C por 15 minutos. Dispensar em placas de Petri.

Especificações Técnicas:

Composição (g/l):

  • Peptona Especial – 10.0;
  • Lactose – 10.0;
  • Extrato de Levedura – 3.0;
  • Cloreto de Sódio – 5.0;
  • Sacarose – 10.0;
  • Vermelho de Fenol – 0.08;
  • Verde Brilhante – 0.0125;
  • Agar – 20.0;

pH Final= 6.9 + 0.1 a 25°C

 

Aparência do Meio:

Preparado: Pó rosa, fluido e homogêneo.

Desidratado: Pó castanho-alaranjado e muito levemente opalescente.

Controle de Qualidade:

Microrganismos                           ATCC             Crescimento                  Características

Salmonella enteriditis                 13076             Bom                               Colônias

Escherichia coli                             25922            Não tão pobre              Colônias amarelo-esverdeadas

Salmonella typhi                          19430            Não tão pobre               Colônias vermelhas

Staphylococcus    aureus            25923           Não tão pobre     

Salmonella typhimurium           14028            Bom                                 Colônias brancas -rosadas

 

Armazenamento:

O pó é muito higroscópico: Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os sinais de deterioração ou contaminação sejam evidentes. Armazenar em placas preparadas a 2-8°C.

 

Descarte:

Não descartar em esgotos e lixos domésticos. O produto deve ser tratado conforme as regulações oficiais administrativas. Soluções em excesso e não recicláveis destinar para empresa responsável. As embalagens contaminadas devem ser eliminadas como produto não utilizado.

Registro ANVISA:

Não aplicável.

Apresentação:

Frasco com 500 gramas.

 

MODELO: K25-610009

MARCA: KASVI

Sinônimos: Brilliant Green Agar, B.P.L.S. Agar, Brilliant Agar Base Modified, Brilliant Green Phenol Red Lactose Sucrose Agar USP; BGA

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