K25-610055

AGAR TRÍPLICE AÇÚCAR FERRO (TSI)

Descrição do produto

Aplicação:

O Agar Tríplice Açúcar Ferro (TSI) é um meio utilizado para diferenciação de enterobactérias de acordo com: fermentação de lactose, glicose e sacarose e produção de sulfito de hidrogênio e dióxido de carbono.

Preparação:

Suspender 64,5 g de pó em 1 litro de água destilada ou deionizada. Aquecer até ferver até que se dissolva completamente. Dispensar em tubos finais. Esterilizar em autoclave a 121°C por 15 minutos. Resfriar os tubos em uma posição inclinada de modo que o fundo tenha pelo menos 3,5 cm de profundidade.

Especificações Técnicas:

Composição                            g/L

Peptona Especial                  20,0

Lactose                                   10,0

Sacarose                                10,0

Cloreto de Sódio                     5,0

Extrato de Bife                        3,0

Extrato de Levedura              3,0

Glicose                                     1,0

Sulfato Férrico                        0,2

Tiossulfato de Sódio              0,3

Vermelho de Fenol                0,025

Agar                                       12,0

pH Final = 7,3 ± 0,2 a 25°C

 

Aparência do Meio:

Desidratado: Pó rosa, fluído e homogêneo.

Preparado: Pó vermelho, levemente opalescente podendo ter um leve precipitado.

 

Controle de Qualidade:

Microorganismos                         ATCC        Crescimento        Ácido        Gás        H2S        

Pseudomonas aeruginosa          9027         Bom                          –               –            –

Salmonella enteritidis                13076         Bom                        +/-              +           +

Escherichia coli                            25922         Bom                        +                +           –

Shigella flexneri                           12022         Bom                       +/-               –           –

 

Armazenamento:

O pó é muito higroscópico: Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os Sinais de deterioração ou contaminação sejam evidentes. Armazenar os tubos preparadas entre 2-8°C.

 

Descarte:

Não descartar em esgotos ou lixos domésticos. O produto deve ser tratado conforme as regulações oficiais administrativas. Soluções em excesso e não recicláveis destinar para empresa responsável. As embalagens contaminadas devem ser eliminadas como produto não utilizado.

Registro ANVISA:

Não aplicável.

Apresentação:

Frasco com 500 gramas.

MODELO: K25-610055

OU

Frasco com 100 gramas.

MODELO: K25-60055

 

MARCA: KASVI

 

Sinônimos: T.S.I Agar, Triple Sugar Iron Agar, T.S.I.

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