K25-610027

AGAR LISINA FERRO (LIA)

Descrição do produto

Aplicação:

É utilizado para diferenciação de microrganismos, especialmente Salmonella (incluindo S. arizona fermentadora de lactose) com base na descarboxilaçao/desaminação de lisina e produção de H2S. A Salmonella fermentadora de lactose irá produzir colônias rosadas no meio contendo lactose.

 

Preparação:

Suspender 34.1g de pó em 1 litro de água destilada ou deionizada. Aquecer até ferver dissolvendo completamente. Dispensar em tubos finais. Esterilizar em autoclave a 121°C por 15 minutos. Deixar os meios solidificar em posição levemente inclinada com base larga.

Especificações Técnicas:

Composição (g/l):

  • Peptona Especial – 5.0
  • Glicose – 1.0
  • Hidrocloreto de L-Lisina – 10.0
  • Citrato Férrico de Amônia – 0.5
  • Extrato de Levedura – 3.0
  • Tiosulfato de Sódio – 0.04
  • Púrpura de Bromocresol – 0.02
  • Agar – 14.5

pH Final = 6.7 ± 0.2 à 25°C

 

Aparência do Meio:

Desidratado: Pó bege, fluído e homogêneo.

Preparado: Pó roxo, levemente opalescente e sem precipitados.

Controle de Qualidade:

Microrganismos                               ATCC                            Fundo                  Inclinação H2S

Escherichia coli                                25922                            Roxo                        Roxo                 –

Salmonella typhimurium               14028                            Roxo                        Roxo                +        

Klebsiella pneumoniae                  13883                             Roxo                        Roxo                 –

Citrobacter freundii                          8090                            Amarelo                   Roxo                  +

Proteus mirabilis                             25993                            Amarelo                   Vermelho         –

 

Armazenamento:

O pó é muito higroscópico: Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os sinais de deterioração ou contaminação sejam evidentes. Armazenar as placas preparadas entre 2-8°C.

 

Descarte:

Não descartar em esgotos ou lixos domésticos. O produto deve ser tratado conforme as regulações oficiais administrativas. Soluções em excesso e não recicláveis destinar para empresa responsável. As embalagens contaminadas devem ser eliminadas como produto não utilizado.

Registro ANVISA:

Não aplicável.

Apresentação:

Frasco com 500 gramas.

 

MODELO: K25-610027

MARCA: KASVI

Sinônimos: MacConkey Agar; MacConkey Agar with 0,15% Bilie Salts, CV and NaCl; MacConkey Agar n°3.

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