K25-610021

AGAR HEKTOEN ENTÉRICO

Descrição do produto

Descrição:

O Agar Hektoen Entérico é um meio seletivo para isolamento e diferenciação de bacilos gram- negativos entéricos. É utilizado para isolar e diferenciar Salmonella e Shigella, as quais causam uma variedade de doenças gastrointestinais sérias em humanos.

Preparação:

Suspender 76.7g de pó em 1 litro de água destilada ou deionizada e deixar absorver por 10 minutos. Aquecer até ferver e misturar até ficar completamente dissolvido. Não superaquecer. Não Autoclavar. Distribuir em placas de Petri.

 

Especificações Técnicas:

Composição (g/l):

  • Peptona Proteica – 12.0;
  • Lactose – 12.0;
  • Extrato de Levedura – 3.0;
  • Sais Biliares n°3 – 9.0;
  • Sacarose – 12.0;
  • Cloreto de Sódio – 5.0
  • Salicina – 2.0;
  • Tiosulfato de Sódio – 5.0;
  • Citrato Férrico de Amônia – 1.5;
  • Fucsina Ácida – 0.1;
  • Azul de Bromotimol – 0.065;
  • Agar – 15.0;

pH Final = 7.5 ± 0.2 a 25°C

Aparência do Meio:

Desidratado: Pó bege púrpuro claro, fluído e homogêneo.

Preparado: Pó verde com fluído amarelo, levemente opalescente.

Controle de Qualidade:

Microrganismos                          ATCC               Crescimento                                Características

Enterococcus faecalis                 29212              Inibido                                         Amarelo

Escherichia coli                            25922              Parcialmente Inibida                Laranja-salmão, pode ter precipitados

Salmonella    typhimurium        14028               Bom                                           Colônias azuis-esverdeadas, com centros pretos

Shigella flexneri                           12022               Bom                                           Colônias azuis-esverdeadas

 

Armazenamento:

Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os sinais de deterioração ou contaminação sejam evidentes. Armazenar em placas entre 2-8°C.

 

Descarte:

O descarte deverá ser realizado de acordo com as normas nacionais e locais vigentes.

Registro ANVISA:

Não aplicável.

Apresentação:

Frasco com 500 gramas.

 

MODELO: K25-610021

MARCA: KASVI

Sinônimos: Hektoen Enteric Agar

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