K25-610019

AGAR EOSINA AZUL DE METILENO (EMB LEVINE)

Descrição do produto

Aplicação:

É um meio seletivo para isolamento de bactérias entéricas Gram-negativas em conformidade com as especificações da Farmacopeia dos Estados Unidos (US). É utilizado em testes de materiais clínicos, alimentos e produtos lacticínios, primariamente para a detecção e confirmação de coliformes.

Preparação:

Suspender 37,5g do pó em 1 litro de água destilada. Aquecer até dissolver completamente. Autoclavar a 121°C por 15 minutos. Resfriar a 45-50°C. Misturar bem. Dispensar em placas de Petri.

Especificações Técnicas:

Composição                                   g/L

Peptona                                        10,0

Lactose                                         10,0

Fosfato Dipotássico                      2,0

Eosina Y                                          0,4

Azul de Metileno                          0,065

Agar                                             15,0

pH Final = 7,2 ± 0,2 a 25°C

 

Aparência do meio:

Desidratado: Pó vermelho calo roxeado fluido e homogêneo.

Preparado: Pó vermelho escuro a azul-roxo e levemente opaco.

 

Controle de Qualidade:

Microrganismos                               ATCC        Crescimento        Características

Escherichia coli                                25922        Bom                     Colônias verdes metálico brilhante

Klebsiella pneumoniae                  13883        Bom                     Colônias rosas

Proteus mirabilis                             25933        Bom                     Colônicas incolores

Pseudomonas aeruginosa             27853        Bom                     Colônicas incolores

Salmonella typhimurium               14028        Bom                     Colônicas incolores

Streptococcus faecalis                   19433        Inibido                         –

 

Armazenamento:

O pó e muito higroscópico: Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os sinais de deterioração ou contaminação sejam evidentes. Armazenar as placas preparadas a 2-8°C fora do alcance de luz.

 

Descarte:

Não descartar em esgotos ou lixos domésticos. O produto deve ser tratado conforme as regulações oficiais administrativas. Soluções em excesso e não recicláveis destinar para empresa responsável. As embalagens contaminadas devem ser eliminadas como produto não utilizado.

Registro ANVISA:

Produto não passível de registro e/ou cadastro.

Apresentação:

Frasco com 500 gramas.

 

MODELO: K25-610019

MARCA: KASVI

 

Sinônimos: EMB Levine Agar, Eosin Methilene Blue Medium, Agar EMB, Agar Eosina Azul de Metileno (Holt-Harris & Teague).

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