K25-610013

AGAR BASE COLUMBIA

Descrição do produto

Aplicação:

É enriquecido com sangue de carneiro estéril (5%) é apropriado para o crescimento e isolamento de microrganismos fastídicos, tais como estreptococos, estafilococos, pneumococos e listeriae a partir de amostras clínicas.

Preparação:

Suspender 43 g de pó em 1 litro de água destilada ou deionizada. Aquecer até ferver até dissolver completamente. Esterilizar em autoclave a 121°C por 15 minutos. Resfrie a 45-50°C e adicionar assepticamente sangue de carneiro desfibrinado estéril 5%. Misturar bem. Dispensar em placas de Petri.

O Agar Columbia Base pode ser enriquecido de diversas formas:

– Com 2 frascos de Suplemento CNA (Staf/Strep) (sulfato de colistina 5 mg/frasco, ácido naledixico 8 mg/frasco, (código K25-81048), cada um reconstituído com 5mL de água destilada estéril. O meio final irá conter sulfato de colistina 10mg/L e ácido naledixico 16 mg/L.

– Com 2 frascos de Suplemento Gardnerella vaginalis (gentamicina 3mg/frasco, anfotericina B 1 mg/frasco, ácido naledixico 15 mg/frasco, código K25-81040), cada um reconstituído com 5 mL de uma solução 1:1 de álcool etílico e água destilada estéril. O meio final irá conter gentamicina 6 mg/L, anfotericina B 2 mg/L e ácido naledixico 30 mg/L.

Especificações Técnicas:

Composição                          g/L

Peptona Especial                23,0

Amido                                     1,0

Cloreto de Sódio                   5,0

Agar                                       14,0

pH Final = 7,3 ± 0,2 a 25°C

 

Aparência do Meio:

Desidratado: Pó bege, fluído e homogêneo.

Preparado: Pó vermelho-cereja e opaco

 

Controle de Qualidade:

Microrganismos                             ATCC        Crescimento        Características

Streptococcus pyogenes              19615         Bom                      Hemólise-β

Streptococcus pneumoniae           6303        Bom                      Hemólise-α

Staphylococcus aureus                25923         Bom                      Hemólise-β

Gardnerella vaginalis                   14018         Bom                      Hemólise-β

 

Armazenamento:

O pó é muito higroscópico. Armazenar entre 10-30°C, em um ambiente seco, em sua embalagem original bem fechada. Utilizar antes da data de validade informada no rótulo ou até que os sinais de deterioração ou contaminação sejam evidentes. Armazenar em placas preparadas a 2-8°C.

 

Descarte:

Não descartar em esgotos ou lixos domésticos. O produto deve ser tratado conforme as regulações oficiais administrativas. Soluções em excesso e não recicláveis destinar para empresa responsável. As embalagens contaminadas devem ser eliminadas como produto não utilizado.

Registro ANVISA:

Produto não passível de registro e/ou cadastro.

Apresentação:

Frasco com 500 gramas.

 

MODELO: K25-610013

MARCA: KASVI

 

Sinônimos: Agar Columbia Sangue Base, Columbia Blood Base.

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